Bonodip Chowdhury

3 Questions 2 Answers 0 Followers

Questions related from Bonodip Chowdhury

Why bacterial cells gradually vanishing from the culture over time?

While growing my bacterial culture in liquid media for protein expression, I noticed that the E. coli cells initially grow well until the OD600 reaches approximately 0.4-0.6, after which they...

13 September 2024 8,790 3 View

What to do when GST- tagged protein is getting precipitated during dialysis?

I purified my recombinant GST-tagged protein in Tris buffer at pH 7.6 (the protein’s PI is 7.11) using a 50 mM reduced glutathione concentration in the elution buffer. However, after dialysis to...

22 May 2024 10,017 2 View

What could be the possible reasons for not occurring cell lysis via sonication during the protein preparation step?

I express my protein in E. coli. After pelleting down the induced medium, I begin lysing the cell pellets through sonication. Suddenly, I am observing that my cells are not lysing. What could be...

14 March 2024 8,136 2 View