Bonodip Chowdhury

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Questions related from Bonodip Chowdhury

What to do when GST- tagged protein is getting precipitated during dialysis?

I purified my recombinant GST-tagged protein in Tris buffer at pH 7.6 (the protein’s PI is 7.11) using a 50 mM reduced glutathione concentration in the elution buffer. However, after dialysis to...

22 May 2024 9,893 2 View

What could be the possible reasons for not occurring cell lysis via sonication during the protein preparation step?

I express my protein in E. coli. After pelleting down the induced medium, I begin lysing the cell pellets through sonication. Suddenly, I am observing that my cells are not lysing. What could be...

14 March 2024 8,051 2 View