Bong-Soo Kim

2 Questions 4 Answers 0 Followers

Questions related from Bong-Soo Kim

Does ECM disppear after ptoeolytic-induced epitope retrieval on immunohistochemistry?

I usually use heat-induced retrieval, but some antigen only works with proteolytic-induced retrieval (PIER). I put the slides into a proteinase K solution, 37C, 5min, and the cartilage matrix...

27 July 2020 8,829 0 View

Any suggestions antigen retrieval methods in immunohistochemistry with bone and cartilage samples?

I am doing IHC with bone and cartilage samples (eg. growth plate). For antigen retrieval, I usually heat the samples in Citrate buffer (pH 6.0) using pressure cooker (for 1 min at max. temp and...

26 February 2018 4,840 8 View