Why my assay for Camptothecin with breast cancer cell cannot observe low viability even though in high concentration above 0.1mM ?

I'm doing for cell surviving rate of breast cancer cell (MDA-MB-453) with Camptothecin (CPT) I used RPMI 1640 for culture MDA-MB-453 (our lab did it for a long time)and seeding to 96 well plate...

09 July 2017 980 7 View