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We used mouse cell line HEI-OC1 and checked OCTN1 expression by WB, then we did IP cell lysate with anti OCTN1 antibody and IB with OCTN1 antibody, surprising! The band is way above IB with WB (30...
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If LC3-I and LC3-II expression both increase in cochlea of aged animal compared to young animal, can we say that autophagy is activated or enhanced? Thanks.
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