*WESTERN BLOT* How do you transfer high and low MW proteins at the same time? I'm trying to get Claudin-5 (~22kDa) and ZO-1 (~220kDa)

I run the electrophoresis in a pre-cast 4-20% gel at 200 V for ~45 min, then i've tried to transfer them to nitrocellulose membrane in the following conditions (always in a cold, wet environment):...

01 January 1970 446 7 View