Why is my lentiviral construct not being packaged?

I received a lentiviral transfer plasmid from a colleague. The plasmid looks like: LTR-Psi-RRE-cPPT-[EcoRI]-PromoterA-Gene-2A-mCherry-[NotI]-WPRE-LTR I digested this plasmid with EcoRI/NotI and...

18 April 2021 3,114 3 View