Bipasa Dey

2 Questions 5 Answers 0 Followers

Questions related from Bipasa Dey

Why my protein is always accompanied with an extra band after Ni NTA?

I am trying to purify a protein via Ni-NTA chromatography. The theoritical size of my protein is 58 kDa which after addition of his tag and thrombin site adds upto 2-3KDa resulting in protein band...

03 July 2023 923 0 View

No colonies after transformation into BL21DE3 pLysS. What could be wrong?

Hello everyone, I am trying to overexpress a protein into BL21DE3 pLysS and have successfully cloned the gene in DH5α and checked the plasmid via double digestion to check the fallout. The...

06 April 2022 3,266 8 View