Bin Lai

5 Questions 26 Answers 0 Followers

Questions related from Bin Lai

How to get TonB knock out mutant?

Dears, Is there any one having any experience with knocking out tonB gene, to disrupt TonB dependent transporting system in bacterial? we constructed the knockout plasmid but could not get any...

09 September 2018 5,591 3 View

Reduction of ferricyanide facilitated by light and oxygen?

Hi, I have been noticed a phenomenon for long that if the ferricyanide solution (in salt buffer) was left open on the bench, the solution would become colorless in hours; but if the solution is...

05 May 2018 6,755 1 View

Which is a suitable buffer to dissolve membrane proteins?

Hi, would any of you advise a good buffer to disolve the membrane protein fractions after TCA or methanol precipitation? The buffer should be compatible for Trypsin digestion and will be better to...

07 July 2015 1,363 6 View

Could anyone advise any sensitive method to measure NAD(P)+ and NADPH?

We tried to do those measurements with a commercial colorimetric assay kit, but finally found its sensitivity does not fit our case. That can only give accurate readings for 10pmol changes which...

11 November 2014 7,325 13 View

What is the solubility of NADH and NADPH in DMSO?

Does anyone know the solubility or have ever done solubility tests of NADH and NADPH in pure DMSO? If soluble, what form are they in, tetra salt?

10 October 2014 2,618 10 View