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Questions related from Bilgenur Baloglu
I am trying to phosphorylate both of my forward and reverse primers prior to the PCR. Primers that are 5' phosphorylated (theoretically) do not affect PCR since primer extension occurs from the...
09 December 2018 6,538 9 View
I have 4 sites with a total of 22 species (i.e. site1 has 7 species, site 2 has 15, etc.). I also have multiple weeks’ species abundance data for each site. I want to analyze the species diversity...
09 May 2016 4,532 3 View
To determine whether the sampling was sufficient, I plotted rarefaction curves for four sites using rarecurve() function in R vegan package. Seems like the curves have not yet "converged" (i.e....
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I am experiencing a contamination problem in my DNA extracts. I did DNA extraction from water samples using phenol/chloroform. However, I do get a shoulder/high peak at 230 nm whereas there is a...
24 October 2014 7,689 5 View
I am working on Sequence Read Archive (SRA) datasets. I have one fasta file containing the SRA identifier and the sequence (atcagtt..) and one blast output file including sequence identifier, gi...
01 September 2014 4,283 11 View
I have an excel file which contains the lines I want to extract from the original file. There are a few issues: 1) that I do not have standardized lines aka identifiers. So I cannot use a command...
15 July 2014 6,064 22 View
I will need to do deep water sampling (preferably at different depths with some number of replicates) firstly in lake and reservoirs and then in marine water. I am currently looking for deep water...
02 April 2014 3,652 3 View
I know that there are several approaches to detect contamination in submitted NCBI transcriptome studies databases, but since I am doing a literature review, I would appreciate if you add up to my...
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