Bilal Khalil

2 Questions 3 Answers 0 Followers

Questions related from Bilal Khalil

Is there a way to isolate nuclei without purifying cytoplasmic protein aggregates?

Hello, I am trying to isolate nuclei from HEK293T cells expressing a protein that aggregates in the cytoplasm, however when I run my samples in western blot the cytoplasmic protein is strongly...

03 November 2020 9,719 3 View

How do i solve my problem with my site-directed mutagenesis experiments?

Hello, I am trying to generate a single mutation in a 10Kb plasmid using Quick Change XL protocol. After the PCR I performed gel electrophoresis and obtained a single band. Then I performed DpnI...

30 July 2015 2,237 9 View