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I´m working with the lipotoxic model in primary mouse hepatocytes (almost all the literature in this fild is with HEPG2). I treated the cells with palmitate 0.5mM during (2-21 hours), and I´m not...
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I would like to know what may be happening in my Western Blot where I didn't see changes in the phosphoprotein (control and stress conditions), but the total protein has disappeared in the stress...
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