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I am trying to isolate primary cells from intervertebral disk. I need 0.03% of collagenase . So I made a stock of 0.2% and stored in -20 degree. After few days later, when I was trying to isolate...
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I am isolating AF cells from porcine. Sometimes it happens that cells are live on the basis of live/dead assay by Calcine AM/ethylene Homo-dimer, but do not attached on plates. I have tested the...
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