Getting shifted band but not able to get supershift with Sp1 or Sp3 antibody. How can I get supershift in EMSA with biotinylated oligoes?

I am doing non-radioactive EMSA with biotinylated oligo. My oligo is GC-rich in sequence. I used to get retarded band in my gel which was successfully competed by cold chase. Other experiments...

16 April 2015 9,165 5 View