Bhumika Bhatt

2 Questions 5 Answers 0 Followers

Questions related from Bhumika Bhatt

I can't detect PKC theta nor ADRP on my westerns. I have tried different antibodies with no luck. Is there a trick to it?

My protocol: lysate in RIPA buffer; 1D in pre-cast 4-12% gradient gel; transfer at 30V over 2 h; block in 8% non-fat milk in tris-buffered saline with 0.1% tween 20 (TBS-T); incubate with primary...

06 June 2014 8,077 5 View

What can be done to minimize clumping and vacuole formation in HepG2 cells grown in DMEM with high glucose and 10% FCS, 1% PenStrep?

I've transferred HepG2 cells from DMEM/F12 to DMEM with high glucose. They look fine in the first 24 hr, then they inevitably start clumping . Any suggestions on how to avoid this?

08 August 2012 8,280 8 View