Has anyone dealt with nonspecific Hoechst staining on the cell surface?

I am trying to stain nuclei of Hela cells using Hoechst 33342. I have tried several working concentrations ranging from 0.2 ug/mL to 1 ug/mL for minimum 2 mins up to 10 mins. I obtain a weird...

21 August 2019 7,133 8 View