What can be done to minimize clumping and vacuole formation in HepG2 cells grown in DMEM with high glucose and 10% FCS, 1% PenStrep?

I've transferred HepG2 cells from DMEM/F12 to DMEM with high glucose. They look fine in the first 24 hr, then they inevitably start clumping . Any suggestions on how to avoid this?

20 August 2012 4,833 7 View