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Questions related from Bhoomika Sharma
I am using Agilent miRNA first strand synthesis kit (cat#600036) for preparing mi cDNA from total RNA. A universal primer is provided with the kit sequence of which is not given. i want to study...
08 August 2018 2,262 2 View
Hello everyone ! I am using mirVana miRNA isolation kit for the isolation of total RNA containing small RNA. Further i used Agilent first strand synthesis kit(cat#600036) for the synthesis of...
08 August 2018 780 5 View
i tried gradient PCR for a new set of primers and got the amplification. However, when i tried running the same set of primers on a single annealing temperature, it showed no or weak...
11 November 2016 9,276 14 View
I am trying to amplify my genes of interest.Though I got the amplified product of the desired size on my first attempt , i am not able to get the amplification again. What might be the problem and...
08 August 2016 9,467 3 View
I have been using mirVana miRNA isolation kit for total rna isolation and had a good experience using that. Recently, i am facing problem with the kit. 280/230 ratio and concentrations are too low...
04 April 2016 5,043 3 View
I am trying to isolate RNA from Breast tumor and adjacent normal tissues (stored in RNAlater at -20 C) using Trizol method. After adding isopropanol , I am getting a transparent droplet like...
03 March 2016 1,462 3 View
I am performing real time pcr for ACTB. I am getting a ct value of 17-20 in my samples. However , i am getting a peak in NTC too with a ct value around 33-35. Also the dissociation curve for NTC...
11 November 2015 3,774 8 View
I am getting two bands below 100 bp in addition to the gapdh amplicon . What might be the reason for these bands? Lane 2- positive control lane 3-6 - samples lane 8- negative control
07 July 2015 4,122 6 View
I am trying to predict miRNAs against the gene of interest using online tools like Targetscan, miRanda ,miRsystem and DIANA micro T- CDS. As these tools produce a long list of miRNAs that might...
07 July 2015 8,384 6 View
I have to perform qPCR for miRNA isolated from colorectal tumor and adjacent normal tissue samples. Is U6 snRNA a good endogenous control for qPCR?
06 June 2015 3,678 9 View
I have downloaded the miRNA seq data for colon adenocarcinoma from TCGA. If I would like to see the expression of a particular miRNA across all the samples, how should we proceed?
04 April 2015 5,551 1 View
Can i use the miRNA Sequence (given in miRBase) as the forward primer or design the stem loop primers?
02 February 2015 467 0 View
I usually collect samples in ice cold PBS. There is approx 30 mins time lag between the collection and the storage of samples. I keep samples in RNAlater at -20C for long term storage. Does this...
02 February 2015 8,006 6 View
