Bhawana Gupta

4 Questions 5 Answers 0 Followers

Questions related from Bhawana Gupta

Has anybody found better anticancer effect on patient derived primary cancer cells than cell line?

I am testing compounds on the patient's primary cancer cells as well as a cell line. Generally, I see better activity in cell lines compared to primary cells. Surprisingly I am seeing the...

19 March 2020 668 3 View

Any body did B CLL cell proliferation assay?

I need to set CLL proliferation assay, but my CLL cells are not proliferating. I am incubating for 72-96 h in IL4 & bME containing RPMI (reduced serum). If anybody knows the trick to grow...

02 November 2017 6,795 3 View

Can anybody please let me know, how to freeze the primary CLL cells?

I need to freeze primary CLL cells. Can anybody tell me the appropriate cell freezing media so that i can get better cell viability at the time of thawing and furthet culturing

11 September 2017 6,247 1 View

Whole blood ROS assay

Has anybody done whole blood ROS assay with luminescent dyes

22 May 2017 3,268 3 View