Bhaskar Bhaskar

2 Questions 14 Answers 0 Followers

Questions related from Bhaskar Bhaskar

Why there is a streak in western blot at a particular molecular weight range , in my case at 60 Kda, while same blot give sharp band at 50 and 70 Kda?

I am getting streaks at 60 Kda, while same blot do not gave any streak at 50 kDa or 70 kDa.? I have repeated it several times, but got the same result. While I do not get any streaks when protein...

06 June 2016 642 6 View

How to avoid protein aggregation during protein concentration?

I am working on a protein which forms oligomers and is functional in oligomerized state. When I tried to concentrate the protein with ambion by centrifugation in 50 mM Tris pH 8.0 with 300 mM...

09 September 2013 5,180 31 View