Beverley Marie Rabbitts

3 Questions 18 Answers 0 Followers

Questions related from Beverley Marie Rabbitts

Any advice on dye labeling of peptide N-termini before electrophoresis?

I would like to label the N-termini of my peptides before electrophoresis. I found GE Healthcare's CyDye DIGE fluors, which can be used in this manner and imaged using the Typhoon scanner, but...

04 April 2015 3,875 5 View

Western to detect MDA-proteins?

Is there an antibody that works well in a western to detect proteins that have been conjugated to malondialdehyde (MDA), the reactive aldehyde lipoxidation product? Could you recommend a good...

08 August 2014 4,513 6 View

What is the most appropriate mitosox staining quantification calculation to use?

I have heard it argued that you should get the maximum pixel intensity (but won't that be prone to artifacts?), because that is the maximim ROS burst. I have also heard you should rather count the...

08 August 2014 6,439 5 View