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Questions related from Bettina Hieronimus
We knocked down a MMP in SK-Mel-28 cell lines and found that STAT3 protein is reduced upon the knock down. Now we are looking for a link between the MMP and STAT3 expression. Does anyone have a...
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I am struggling with the method for subcellular membrane fractioning. I use Optiprep and tried continuous and discontinous gradients 1-25% and 10-40% with human melanoma cells. The method was run...
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We try to precipitate the protein from cell culture media after PMA stimulation. Protocol is as follows: - starve the cells (MDCK) for 1h with DMEM +0,2% BSA - stimulate 30 min with 0-50ng/ml PMA...
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