Bethany Coull

2 Questions 2 Answers 0 Followers

Questions related from Bethany Coull

How to serum starve the cell lines bEnd.3 and sEnd.1 (middle t antigen expressing)?

I've been working with bEnd.3 and sEnd.1 cells which are endothelial cell lines from the periphery (sEnd1.s) and the brain (bEnd.1s). They are immortalised using middle T antigen. I need to serum...

23 March 2018 9,321 3 View

Help with primary brain endothelial cell isolation from mice?

I've been trying to isolation brain endothelial cells from mouse brain. Protocol that I've tweaked from one I found on bioprotocol: Cull via cervical dislocation, remove brain and put in ice cold...

21 February 2018 3,736 3 View