Bertha Mak

5 Questions 8 Answers 0 Followers

Questions related from Bertha Mak

Co-localization analysis tools for confocal images?

Hi, I am currently working on the colocalisation of 2 proteins and I plan to work it out with confocal microscopy. In the school I am working we have the leica LasX software with the...

26 April 2021 2,944 9 View

Seeing signal from Alexa Fluor 594 under 488 excitation ?

I am currently working on protein co-localization with confocal microscopy with 2 proteins, let's call them protein A and protein B. Protein A is located mostly in cytoplasm with little amount in...

14 April 2021 1,261 5 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

17 February 2021 9,371 5 View

Nuclear lysis method for Co-immunoprecipitation ?

I am currently working on a kinase protein which is only present within the nucleus and need to perform a Co-IP to confirm its binding with another protein. I have consider a lot of lysis method...

07 January 2021 5,569 4 View

Desperate help for awkward confocal images. Problem with antibodies?

Hi all, I am in desperate need for my IF experiment and I am suspecting there's problem with my primary antibody (which I really hope I am wrong). Please find the 3 pictures attached and pay...

01 January 1970 5,997 5 View