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Questions related from Bertha Mak
Hi, I am currently working on the colocalisation of 2 proteins and I plan to work it out with confocal microscopy. In the school I am working we have the leica LasX software with the...
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I am currently working on protein co-localization with confocal microscopy with 2 proteins, let's call them protein A and protein B. Protein A is located mostly in cytoplasm with little amount in...
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Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...
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I am currently working on a kinase protein which is only present within the nucleus and need to perform a Co-IP to confirm its binding with another protein. I have consider a lot of lysis method...
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Hi all, I am in desperate need for my IF experiment and I am suspecting there's problem with my primary antibody (which I really hope I am wrong). Please find the 3 pictures attached and pay...
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