Benjamin P. Garfinkel

2 Questions 3 Answers 0 Followers

Questions related from Benjamin P. Garfinkel

No plasmid following gateway cloning, even though DH5a are antibiotic resistant?

Hi, We have years of experience cloning, but are encountering a baffling situation we can't figure out. This is happening following LR-recombinase cloning. We transform DH5a cells, and plate on...

08 August 2019 5,526 4 View

QPCR of pri-miR?

Hi, does anyone have experience with analysis of pri-miRs by qPCR? Can I simply design primers like I would for a gene (and place them flanking the hairpin)? I see there is a very expensive taqman...

09 September 2016 2,428 3 View