Benjamin Unruh

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Questions related from Benjamin Unruh

Is it possible to "simulate" a high-fat diet in Cell Cultures? Has anyone done it?

Hello, I have not found much information on this, but I was curious if anyone has ever tried to supplement cholesterol and fatty acids instead of just using FBS and used this to simulate a...

19 December 2018 6,279 3 View

I am staining mouse liver with a primary mouse antibody, how do I eliminate non-specific bands in my secondary antibody?

Hello, I extracted mouse liver tissue using triton-x-1% with several different protease and phosphatase inhibitors. I did the same thing with NIH3T3 cells and AML12 cells. When I detect actin,...

24 August 2018 7,624 6 View