Benjamin Thomas

3 Questions 1 Answers 0 Followers

Questions related from Benjamin Thomas

Viability assessment: multiple fluorescent dye staining of adipose tissue (to be fixated & sectioned) with CalceinAM (green) Hoechst (blue) DiI (red)?

Dear fellow researchers: I am looking for the best way to visualize adipocytes, which have been transferred from one mouse to another, and assess cell viability. Volumes of 1-2 ml are obtained...

21 February 2018 3,654 1 View

Best way to label adult adipocytes & pre-adipocytes in rodents (particularly mice) obtained by liposuction/enzymatic isolation before seeding/culture?

Hey folks. I was wondering what the best way was to label adult adipocytes (which I do not expect to divide any further) and pre-adipocytes (dividing further) without changing their biological...

26 November 2017 2,521 1 View

Is HE staining still possible after sectioning and mounting slides a few years ago (they were stored at room temperature)?

Hey folks, I have a batch of slides with histological cross sections which were originally embedded in paraffin. They were then sectioned and mounted between 2 and 3 years ago. Now we want to do...

24 August 2017 2,882 14 View