Bengisu Molyer

2 Questions 5 Answers 0 Followers

Questions related from Bengisu Molyer

I only get empty vector when cloning, what could be the problem?

Hello everyone I'm trying to pseudotype an oncolytic virus by restriction enzyme cloning. I have generated my insert by PCR by adding the necessary cutsites. I only have one restriction enzyme...

05 May 2019 3,841 10 View

3kb plasmid insert amplification by pcr?

Hello everyone, i am trying to amplify a 3kb insert with 64% GC content from a plasmid to be used for downstream cloning purposes. My lab does not do much cloning so we only have SYBR green taq as...

01 January 2019 4,917 3 View