Bengisu Molyer

3 Questions 4 Answers 0 Followers

Questions related from Bengisu Molyer

Monocyte derived M0 macrophages do not fit flow cytometry scale?

Hello everyone I am differentiating MDM form human PBMC using M-CSF for 6 days. My problem is that when I read my macrophages on the cytoflex for flow cytometry, they do not fit the forward...

05 February 2023 4,662 5 View

Human Osteosarcoma (HOS) cell transfection with Lipofectamine 2000?

Hello everyone, I am trying to do a viral rescue by transfecting my viral plasmids into HOS cells using Lipofectamine 2000. I was not able to rescue my virus so I was wondering if anyone has any...

24 October 2019 8,208 0 View

I only get empty vector when cloning, what could be the problem?

Hello everyone I'm trying to pseudotype an oncolytic virus by restriction enzyme cloning. I have generated my insert by PCR by adding the necessary cutsites. I only have one restriction enzyme...

28 May 2019 2,590 9 View