I designed a construct containing an IRES to co-express two genes. I only get expression from the first gene (5' of IRES). What might be the problem?

I designed the following construct: [5' PCMV_MCS1_IRES_MCS2 3']. I am only able to express genes I clone into MCS1. The genes I clone into MCS2 are not expressed. What could be the problem?

25 August 2015 6,558 14 View