Benedetta Colmegna

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Questions related from Benedetta Colmegna

Which concentration of collagenase (unit/ml) can I use to dissociate human cancer samples?

I would like to obtain primary culture from mesothelioma and ovarian cancer samples. Which concentration of collagenase (unit/ml) can I use for the dissociation of these tissues? And incubation...

31 January 2017 2,259 3 View

Can anyone suggest me how can I test the activity of Wnt-3A conditioned medium with the top fop assay?

I'm using cell to produce Wnt-3a conditioned medium to growth organoid. How can I measure the activity of the medium ? I also would like to check the performance of my cells . Do they have limited...

04 October 2016 9,184 2 View