Bellamkonda Ramakrishna

4 Questions 1 Answers 0 Followers

Questions related from Bellamkonda Ramakrishna

Can i use the crude enzyme extraction supernatant (in PB buffer pH7.5) for different enzyme assays?

I would like to assay PAL, PPO, CAT and SOD in rice seedlings. I extracted the enzymes in phosphate buffer pH7.5. But, PAL has its activity at pH8.8 , and POD at pH 6.5 , CAT at pH 7. Can i use...

09 September 2016 2,938 6 View

How to grow rice seedlings in hydroponics/ vermiculite/ acid wash sand?

some authors using Half strength Hogland/ MS and others using full strength. What is the rationale to select the half strength and full strength. Please give me your suggestions to grow rice...

08 August 2016 211 1 View

How to perform enzyme activity assays?

If i want to perform any enzyme activity how to select the substrate concentration in 3 mL reaction mixture? and if there is any donor is present in 3 mL mixture how to select the concentration of...

08 August 2016 581 3 View

How to prepare origami competent cells?

Should i need to add antiboitcs ? if so what is the concentrations?

07 July 2016 5,119 2 View