Beitnere Ulrika

2 Questions 8 Answers 0 Followers

Questions related from Beitnere Ulrika

How much protein (meaning concentration mg/ml) do you mostly get out of a rat hippocampus?

Hi Western friends! I'm using cold RIPA buffer with a protease inhibitor cocktail (200 ul for each hippocampus) and I'm homogenising each sample for about 8 seconds with the homogeniser IKA T10B...

11 November 2014 4,960 9 View

What could be the problem with my Coomassie blue?

I got a really messy SDS-PAGE gel colored after transfer with Coomassie blue. I'm wondering also why the sample stopped running although the blue line with the samples was much lower than the...

09 September 2014 2,169 10 View