How to distinguish the 20 bp band length (393 and 374 bp) in gel electrophoresis?

I am trying to distinguish between 393 and 374 band lengths by RFLP method. I am running in 3.5% agarose gel in 25x15cm gel electrophoresis to visualize the enzyme cut. I could not distinguish the...

27 February 2024 8,175 8 View