Bedabrata Saha

3 Questions 5 Answers 0 Followers

Questions related from Bedabrata Saha

What is the exact procedure of cloning a 4 kb gene from a plant source?

I am trying to clone a 4kb known gene from a plant source using High Fidelity Taq polymerae but unable to get the desired product. Please suggest what pin points should be taken care off, to get...

05 May 2016 7,872 9 View

Why don't transgenic plants give reverse transcriptase PCR for which they are genetically engineered?

I am having a problem getting Reverse Transcriptase PCR (for which the plant is genetically engineered) especially in progeny lines, even after selecting in stress conditions. These plants are PCR...

12 December 2013 742 9 View

Hybridization and washing temperature for southern hybridization?

I would like to know how to calculate the Tm of my probe and thus find out the exact and perfect hybridization and washing temperature if I am using formamide in my hybridization buffer. I have...

06 June 2013 1,754 3 View