Becky Bamford

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Questions related from Becky Bamford

Click chemistry - how to eliminate unspecific binding of TAMRA alkyne?

I am labelling myristoylated proteins in cells and performing a click chemistry reaction to add the fluorescent TAMRA-alkyne to visualise via in-gel fluorescence. In a negative control sample...

29 March 2021 1,129 1 View

Promega TNT in vitro translation system - Is it possible to use 2 radio labelled amino acids to enhance efficiency?

Hello, I am using Promega's TNT in vitro coupled transcription/translation system with radio labelled 35S methionine and getting low to no yield with some of my proteins that I'm trying to...

11 March 2019 7,270 1 View