What is the minimun time of fixation using an in-situ Hybridization protocol in whole mouse embryos?

I would like to know if someone has made an in-situ in tissues fixed during 1h in PFA. We usually fix the tissue in PFA 4% O/N, but we have some samples stored in MetOH after a short fixation in...

09 September 2017 2,699 3 View