I'm performing Western Blot for Ki67 (345 kDa) in cell lysate but can't see bands. Any advice on protocols or protein concentrations would be to know?

I am performing Western Blot to detect the Ki67 proprotein in cell lysate, which has a molecular weight of 345 kDa. I am unable to see bands after transfer. I would appreciate any advice on...

20 January 2025 1,702 3 View