Beatriz Dias Barbieri

5 Questions 9 Answers 0 Followers

Questions related from Beatriz Dias Barbieri

Why is my RNA IVT product shorter than expected?

Hi all, I have been trying to synthesize luciferase self-amplifying RNA (saRNA) by in vitro transcription (IVT), and this vector has approximately 11kb. The main problem is that I am getting a...

07 March 2023 582 7 View

How to calculate N/P ratio?

Hi all, I am working with lipid nanoparticles encapsulated with RNA and I need to calculate the N/P ratio for their preparation. However, the literature is not very clear on how to calculate it....

12 April 2021 6,352 4 View

How to dissolve lipid in ethanol if stock comes in oil?

Hi all, We purchased a lipid recently and the company ships it in liquid oil. However, we would like to resuspend it in ethanol to be able to mix with other lipids already dissolved in...

10 March 2021 2,523 1 View

Are there any differences between setting automatic or manual number of measurements in DLS?

When doing measurements in DLS (either size or zeta potential), I have the option to input an automatic number of measurements or to set it manually. My question is: is there a drawback in...

09 February 2021 9,599 6 View

For how long can I store lipids (in solution) in -20°C freezer?

Hi everyone, I am preparing liposomes and I would like to know for how long lipids can be stored in a -20 °C freezer, please? At the moment, I am working with DOTAP, DOPE, Cholesterol, and PEG,...

03 February 2021 7,280 7 View