Problem with cloning: why positives clones of colony PCR not giving desired amplicon in PCR from purified plasmid?

We cloned our gene of interest in pcDNA3.1(-) in between EcoRI and XhoI. In colony PCR (with primers used for amplify gene of interest), we got distinct amplicon of desired length. However, we...

02 February 2019 4,679 6 View