Bassam Alkotaini

3 Questions 7 Answers 0 Followers

Questions related from Bassam Alkotaini

How can I get a pure eluted His-tag protein ?

I have successfully expressed a soluble fraction of an enzyme after having heat shock proteins co-expressed with it. Cells were broken by sonication and further centrifuged. The cell lysate was...

10 October 2015 9,852 6 View

Why does cellulase enzymes are not expressed in E.coli BL21(DE3)?

I am using pTD-NStrep-His vector. I have cloned two genes in two individual vectors. Those genes are two cellulase enzymes. I used Gibson kit during the cloning process and all results were great...

05 May 2015 2,493 4 View

Can the glycolytic cycle be carried out using the cells extract (containing certain enzymes) and the glucose as a substrate for the reaction?

Can we do this reaction outside the cell using the cell extract ??

03 March 2014 6,597 4 View