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I use the santa cruz C4 sc-47778 B actin anti-body as the reference in my blots. When i load only 3 samples on my gel, the antibody seems to bind to something and it shows as if i loaded the...
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my samples are very concentrated ie for 30 ug/mL i add an average of 9 uL of my protein sample add 2.5 uL sample buffer and 1 uL sample reducer and i complete to 35 uL with ultra-pure water. Now,...
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