What should I do to get over on smear and leakage and why the protein marker separated like that?

I am working on the brain tissue of mice (10% SDS-PAGE gel). The protein sample was extracted with lysis buffer (Tris-HCL, Triton, and B-mercaptoethanol). I think this leakage is due to the...

22 December 2023 2,264 2 View