Basem M. Ahmed

3 Questions 8 Answers 0 Followers

Questions related from Basem M. Ahmed

Optimising RNA yield (quantity and purity) for next generation sequencing?

RNA extraction from egg allantoic fluid with high virus titer using trizol yielded from 350 to 60 ng/ul RNA but the purity is 1.4. I managed to transfer trizol aquous phase into qiamp viral kit...

07 July 2019 9,402 3 View

Is it normal to have a plasmid in chemically competent Max effeciency DH5 Alpha T1?

working on cloning of some viral genes into PfastBac1, vast amount of unexpected results directed me to insult the competent cells, so I had propagated it and performed miniprep, plasmid is found,...

12 December 2014 8,769 5 View

I want an excellent freeware for detecting probable active sites in a protein sequence, suggestions please?

I have some deduced sequences for a specific protein from diseased as well as apparently healthy animals, I just want to detect active sites and see differences and their effect if any.

04 April 2014 5,265 6 View