Basant Hamdy

4 Questions 14 Answers 0 Followers

Questions related from Basant Hamdy

Any advice on cultured fibroblast cell density?

GreetingsI have plated 50,000 fibroblast cell/ml in a 60 mm dish & around 5000 cell/ml in a 12 well plate, since about 5 days.I'm monitoring them & they still didn't attain a monolayer...

06 June 2015 3,567 12 View

Any recommendation for harvesting cells from 12 well plate?

Greetings Can anyone suggest a technique for harvesting adherent fibroblasts from 12 well plate with minimal strain to the cells?

06 June 2015 6,915 7 View

Is the behavior of normal keratinocytes transformed by HPV proteins different from non_transformed normal keratinocytes?

The question is about their behavior in cell culture models. Considering cell proliferation & expression of certain invasion-related markers. Thank you

12 December 2014 8,604 6 View

What is the recommended RNA extraction protocol from cultured adherent human cell lines?

I'm planning for culturing SCC cell line & want to know the best technique to extract RNA from them & recommended protocol or extraction kit prior proceeding qPCR steps. I'm still confused...

12 December 2014 1,315 16 View