Help troubleshooting failed phage transduction?

Lysate prep: donor strain is inoculated overnight in LB then diluted 1:100 into 5 mL LB + 0.2% glucose + 5 mM CaCl2, incubated for 1 hour. 100 uL of P1vir was added, then incubated until total...

24 September 2021 8,025 4 View