How to express the envelope glycoprotein E2 of Japanese encephalitis virus cloned in pET28a vector?

I have cloned the E2 gene of JEV in BamHI and HindIII sites of pET28a vector along with start codon ATG. Tried to induce the protein with several concentrations of IPTG ranging from 0.2 to 1 mM,...

08 June 2016 2,228 3 View