Barbara Zambelli

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Questions related from Barbara Zambelli

What are some programs to predict circular dichroism spectra?

Does someone know which program I can use to 1) predict a CD spectrum given a PDB structure, 2) predict a CD spectrum on the basis on a secondary structure prediction, and 3) predict the CD...

11 November 2013 6,580 7 View

Does anyone have references for biochemical and structural studies on proteins that retained a (quite large) fusion tag?

We are purifying a protein that is insoluble when expressed as native sequence and in the presence of His-tag. We found a couple of tags, DsbA and ZZ, which are able to make it expressing in the...

11 November 2013 6,616 4 View

Protocols of protein refolding?

I have a protein that is expressed in inclusion bodies (E. coli expression) and I have solubilized it in 6 M GuHCl. I know different methods for protein refolding (dialysis, dilution or in column)...

09 September 2013 1,564 2 View