Failed expression of recombinant nucleation peptide, due to a short spacer between T7 promoter to RBS?

hi all, i'm having a problem to express my gene in E.Coli (BL21) of a nucleation peptide. nowadays i'm suspecting that it might be because of a too short spacer in my vector: (the "map", and the...

06 June 2018 5,324 6 View