Bappi Sarkar

4 Questions 7 Answers 0 Followers

Questions related from Bappi Sarkar

What is the difference between PBS with and without KCl? Is there any point?

I have just started to prepare a PBS buffer for my experiment and I found recipes for a PBS buffer both with and without KCl. What is the difference between both of them?

14 November 2021 9,788 2 View

Problem with GST-fused Construct Induction- Can anyone help?

I am trying to Induce a construct (1.3kb) from Drosophila in the pGEX-4T1 vector in BL21 cells with IPTG. IPTG induction was done in 3hr grown culture and induce for 3 to 4 hr at 37*C. But there...

06 March 2021 2,520 4 View

Problem with GST-fusion protein elution- Can anyone help?

I’m trying to pull down and purify GST tagged protein (target sequence is inserted in pGEX4T1 vector) from BL21 Cell lyset. Target Protein expresses in BL21 cells in good amount, confirmed by SDS...

19 December 2020 4,762 6 View

Can I use Triton X 100 in place of tween 20 during western blotting? If yes then what percentage should I use?

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01 January 1970 8,083 2 View