Bapi Mandal

3 Questions 6 Answers 0 Followers

Questions related from Bapi Mandal

Issue with Protein expression and recovery?

Hello everyone, I seek a little assistance with my stubbornly insoluble protein. I express my protein in E.coli BL21 (DE3) cells with a suitable tag for solubilization. To be precise, my...

31 May 2018 8,547 7 View

I need to amplify a fragment using primers with high GC content (70%, Tm= 76). I tried gradient (58 - 75) pcr, but got no bands. Any Suggestions?

I need to amplify a 2kb gene from the genome. The details of the primers are as follows: FP - GC - 70%, Tm - 76o C RP - GC - 73%, Tm - 77o C

23 September 2016 2,606 7 View

Why No Band Observed After Gradient PCR?

My culture is Lactobaccilus delbrueckii NCDC 253 which is claimed exact to Lactobaccilus delbrueckii ATCC 11842 but after gradient PCR for beta-galactosidase gene (3.2kb) I am not getting any...

16 July 2016 8,049 6 View